Chiral Analysis of Pantolactone with Molecular Rotational Resonance Spectroscopy
Originally Published in Chirality
Authors: Reilly E. Sontrom; Justin L. Neill; Alexander V. Mikhonin; Reinhard Doetzer; Brooks H. Pate
Publication Summary
Originally published in Chirality, this publication presents a molecular rotational resonance (MRR) spectroscopy method for measuring the enantiomeric excess and absolute configuration of pantolactone, an important intermediate used in the production of panthenol and pantothenic acid (vitamin B5). The study demonstrates how chiral tagging enables the differentiation of pantolactone enantiomers by forming diastereomeric complexes with distinct rotational spectra, providing a quantitative approach to chiral analysis without requiring reference samples of known enantiopurity.
The researchers evaluated both broadband and targeted MRR spectroscopy approaches, showing that targeted measurements can deliver reliable enantiomeric excess analysis with sample-to-sample cycle times of approximately 15 minutes. The methodology was validated against established chiral gas chromatography techniques, demonstrating strong quantitative agreement while offering a rapid and streamlined alternative for routine chiral characterization.
Key Takeaways
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MRR spectroscopy enables quantitative measurement of pantolactone enantiomeric excess, supporting accurate chiral characterization of an important pharmaceutical intermediate.
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Chiral tagging allows enantiomers to be distinguished through their unique rotational spectra, enabling both enantiomeric excess and absolute configuration determination.
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The method can be performed without reference samples of known enantiopurity, simplifying analytical workflows.
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Targeted MRR measurements reduce analysis time, achieving sample-to-sample cycle times of approximately 15 minutes.
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Results showed strong agreement with traditional chiral gas chromatography methods, validating the accuracy of the approach.
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The study highlights the potential of MRR spectroscopy as a practical tool for routine chiral analysis in pharmaceutical and chemical applications